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BIOSUPPLY UK LIMITED trap-5b acth eia
Fasted rats were given a single dose of Dexamethasone (15 or 100 µg/kg i.p.) with or without 1.0 µg/kg elcatonin (eCT). Blood was collected by venepuncture and glucose was quantified: i) immediately prior to fasting, ii) prior to drug treatment, and iii) 5 h after drug treatment. Overnight fasting caused a fall in mean blood glucose from 6.19±0.10 to 4.80±0.13 mM. Blood glucose in the vehicle-treated group continued to drop, reaching 3.86±0.22 mM at the 5-hour time point. (A) Blood glucose data as measured 5 h post-treatment, and normalised for differing pre-fasting levels. (B) <t>ACTH</t> was assayed by <t>EIA</t> in blood collected by terminal cardiac puncture at 5 h post-treatment. ACTH suppression is expressed in relation to the levels quantified by ELISA in vehicle-treated animals (1.57±0.09 ng/ml). In both panels, data are mean ± SEM of 8 rats per group. Statistical analyses by one-way ANOVA (Kruskal-Wallis test with Dunn’s post-test); *p<0.05, **p<0.01, and ***p<0.001 as compared to vehicle-treated group, or by Mann-Whitney test; † p>0.05 in the indicated comparison.
Trap 5b Acth Eia, supplied by BIOSUPPLY UK LIMITED, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/trap-5b+acth+eia/trap+5b+acth+eia/pmc03564948-61-35-38
Average 90 stars, based on 1 article reviews
trap-5b acth eia - by Bioz Stars, 2026-09
90/100 stars

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1) Product Images from "The Calcitonin and Glucocorticoids Combination: Mechanistic Insights into Their Class–Effect Synergy in Experimental Arthritis"

Article Title: The Calcitonin and Glucocorticoids Combination: Mechanistic Insights into Their Class–Effect Synergy in Experimental Arthritis

Journal: PLoS ONE

doi: 10.1371/journal.pone.0054299

Fasted rats were given a single dose of Dexamethasone (15 or 100 µg/kg i.p.) with or without 1.0 µg/kg elcatonin (eCT). Blood was collected by venepuncture and glucose was quantified: i) immediately prior to fasting, ii) prior to drug treatment, and iii) 5 h after drug treatment. Overnight fasting caused a fall in mean blood glucose from 6.19±0.10 to 4.80±0.13 mM. Blood glucose in the vehicle-treated group continued to drop, reaching 3.86±0.22 mM at the 5-hour time point. (A) Blood glucose data as measured 5 h post-treatment, and normalised for differing pre-fasting levels. (B) ACTH was assayed by EIA in blood collected by terminal cardiac puncture at 5 h post-treatment. ACTH suppression is expressed in relation to the levels quantified by ELISA in vehicle-treated animals (1.57±0.09 ng/ml). In both panels, data are mean ± SEM of 8 rats per group. Statistical analyses by one-way ANOVA (Kruskal-Wallis test with Dunn’s post-test); *p<0.05, **p<0.01, and ***p<0.001 as compared to vehicle-treated group, or by Mann-Whitney test; † p>0.05 in the indicated comparison.
Figure Legend Snippet: Fasted rats were given a single dose of Dexamethasone (15 or 100 µg/kg i.p.) with or without 1.0 µg/kg elcatonin (eCT). Blood was collected by venepuncture and glucose was quantified: i) immediately prior to fasting, ii) prior to drug treatment, and iii) 5 h after drug treatment. Overnight fasting caused a fall in mean blood glucose from 6.19±0.10 to 4.80±0.13 mM. Blood glucose in the vehicle-treated group continued to drop, reaching 3.86±0.22 mM at the 5-hour time point. (A) Blood glucose data as measured 5 h post-treatment, and normalised for differing pre-fasting levels. (B) ACTH was assayed by EIA in blood collected by terminal cardiac puncture at 5 h post-treatment. ACTH suppression is expressed in relation to the levels quantified by ELISA in vehicle-treated animals (1.57±0.09 ng/ml). In both panels, data are mean ± SEM of 8 rats per group. Statistical analyses by one-way ANOVA (Kruskal-Wallis test with Dunn’s post-test); *p<0.05, **p<0.01, and ***p<0.001 as compared to vehicle-treated group, or by Mann-Whitney test; † p>0.05 in the indicated comparison.

Techniques Used: Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

Related Articles

Enzyme-linked Immunosorbent Assay:

Article Title: The Calcitonin and Glucocorticoids Combination: Mechanistic Insights into Their Class–Effect Synergy in Experimental Arthritis
Article Snippet: The following markers were assayed using commercially available kits: MMP-2, CXCL5, CCL5, CCL20, CXCL7 ELISA from R&D Systems (Abingdon, UK); telopeptide of type-I collagen (CTX-I) EIA from Immunodiagnostic Systems (Tyne & Wear, UK); TRAP-5b and ACTH EIA from Biosupply UK Ltd. (Bradford, UK).

MANN-WHITNEY:

Article Title: The Calcitonin and Glucocorticoids Combination: Mechanistic Insights into Their Class–Effect Synergy in Experimental Arthritis
Article Snippet: The following markers were assayed using commercially available kits: MMP-2, CXCL5, CCL5, CCL20, CXCL7 ELISA from R&D Systems (Abingdon, UK); telopeptide of type-I collagen (CTX-I) EIA from Immunodiagnostic Systems (Tyne & Wear, UK); TRAP-5b and ACTH EIA from Biosupply UK Ltd. (Bradford, UK).



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BIOSUPPLY UK LIMITED trap-5b acth eia
Fasted rats were given a single dose of Dexamethasone (15 or 100 µg/kg i.p.) with or without 1.0 µg/kg elcatonin (eCT). Blood was collected by venepuncture and glucose was quantified: i) immediately prior to fasting, ii) prior to drug treatment, and iii) 5 h after drug treatment. Overnight fasting caused a fall in mean blood glucose from 6.19±0.10 to 4.80±0.13 mM. Blood glucose in the vehicle-treated group continued to drop, reaching 3.86±0.22 mM at the 5-hour time point. (A) Blood glucose data as measured 5 h post-treatment, and normalised for differing pre-fasting levels. (B) <t>ACTH</t> was assayed by <t>EIA</t> in blood collected by terminal cardiac puncture at 5 h post-treatment. ACTH suppression is expressed in relation to the levels quantified by ELISA in vehicle-treated animals (1.57±0.09 ng/ml). In both panels, data are mean ± SEM of 8 rats per group. Statistical analyses by one-way ANOVA (Kruskal-Wallis test with Dunn’s post-test); *p<0.05, **p<0.01, and ***p<0.001 as compared to vehicle-treated group, or by Mann-Whitney test; † p>0.05 in the indicated comparison.
Trap 5b Acth Eia, supplied by BIOSUPPLY UK LIMITED, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/trap-5b+acth+eia/trap+5b+acth+eia/pmc03564948-61-35-38
Average 90 stars, based on 1 article reviews
trap-5b acth eia - by Bioz Stars, 2026-09
90/100 stars
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Fasted rats were given a single dose of Dexamethasone (15 or 100 µg/kg i.p.) with or without 1.0 µg/kg elcatonin (eCT). Blood was collected by venepuncture and glucose was quantified: i) immediately prior to fasting, ii) prior to drug treatment, and iii) 5 h after drug treatment. Overnight fasting caused a fall in mean blood glucose from 6.19±0.10 to 4.80±0.13 mM. Blood glucose in the vehicle-treated group continued to drop, reaching 3.86±0.22 mM at the 5-hour time point. (A) Blood glucose data as measured 5 h post-treatment, and normalised for differing pre-fasting levels. (B) ACTH was assayed by EIA in blood collected by terminal cardiac puncture at 5 h post-treatment. ACTH suppression is expressed in relation to the levels quantified by ELISA in vehicle-treated animals (1.57±0.09 ng/ml). In both panels, data are mean ± SEM of 8 rats per group. Statistical analyses by one-way ANOVA (Kruskal-Wallis test with Dunn’s post-test); *p<0.05, **p<0.01, and ***p<0.001 as compared to vehicle-treated group, or by Mann-Whitney test; † p>0.05 in the indicated comparison.

Journal: PLoS ONE

Article Title: The Calcitonin and Glucocorticoids Combination: Mechanistic Insights into Their Class–Effect Synergy in Experimental Arthritis

doi: 10.1371/journal.pone.0054299

Figure Lengend Snippet: Fasted rats were given a single dose of Dexamethasone (15 or 100 µg/kg i.p.) with or without 1.0 µg/kg elcatonin (eCT). Blood was collected by venepuncture and glucose was quantified: i) immediately prior to fasting, ii) prior to drug treatment, and iii) 5 h after drug treatment. Overnight fasting caused a fall in mean blood glucose from 6.19±0.10 to 4.80±0.13 mM. Blood glucose in the vehicle-treated group continued to drop, reaching 3.86±0.22 mM at the 5-hour time point. (A) Blood glucose data as measured 5 h post-treatment, and normalised for differing pre-fasting levels. (B) ACTH was assayed by EIA in blood collected by terminal cardiac puncture at 5 h post-treatment. ACTH suppression is expressed in relation to the levels quantified by ELISA in vehicle-treated animals (1.57±0.09 ng/ml). In both panels, data are mean ± SEM of 8 rats per group. Statistical analyses by one-way ANOVA (Kruskal-Wallis test with Dunn’s post-test); *p<0.05, **p<0.01, and ***p<0.001 as compared to vehicle-treated group, or by Mann-Whitney test; † p>0.05 in the indicated comparison.

Article Snippet: The following markers were assayed using commercially available kits: MMP-2, CXCL5, CCL5, CCL20, CXCL7 ELISA from R&D Systems (Abingdon, UK); telopeptide of type-I collagen (CTX-I) EIA from Immunodiagnostic Systems (Tyne & Wear, UK); TRAP-5b and ACTH EIA from Biosupply UK Ltd. (Bradford, UK).

Techniques: Enzyme-linked Immunosorbent Assay, MANN-WHITNEY